产品本体筛检方法及其在变型没计中的应用

提出了一种应用于产品变型设计中的本体筛检方法,该方法以完整的产品资源本体视图为基础,将产品的功能需求、结构特征等转化为需求约束,设计人员根据需求约束对产品资源本体进行筛检。针对不同的筛检层次和筛检对象,给出了模式匹配、实例匹配与人工决策相结合、实例推理自动匹配等筛检规则。这种人机结合的半自动筛检方法提高了产品变型设计方案的准确性,增加了设计过程的柔性和效率。开发了一套针对建筑门窗产品的变型设计支持系统,证明所述方法的可行性和有效性。     

PCR法快速鉴定阳性克隆实验的改进

分析了常规PCR扩增鉴定阳性克隆实验中存在的问题,进行了改进探索:反转录PCR(RT-PCR)获得目的基因片段,TA克隆连接质粒载体、转化大肠杆菌感受态细胞;利用目的基因的特异性引物,用菌液PCR法直接筛选重组克隆,在筛选的阳性菌液中扩增到与目的基因片段大小一致的阳性条带,与质粒PCR及酶切的阳性对照一致,验证了菌液PCR具有可靠性。实验证明,自身引物菌液PCR法用于定性筛选重组阳性克隆,是一种简便、快速、有效的方法。     

大白菜(Brassica pekinensis L.)对镉富集基因型差异的研究

研究了15种大白菜基因型对镉(Cd)富集的基因型差异,以期筛选出低积累Cd的白菜基因型.盆栽试验结果表明,不同白菜基因型的Cd含量、富集系数(BFs)和转运系数(TFs)差异显著(P0.05).丰源新3号、现代夏秋和春秋六轮的Cd积累量均小于0.50mg/kg,其富集系数和转运系数都小于1.0,具有一定低积累Cd的潜力.大田试验的结果表明丰源新3号的Cd含量小于0.50mg/kg,富集系数和转运系数均小于1.0,表明其为排斥Cd的白菜基因型.现代夏秋和春秋六轮的富集系数和转运系数均小于1.0,但其地上部Cd含量均高于0.50mg/kg,不属于Cd低积累白菜.值得注意的.北京小杂56在盆栽和大田中的Cd含量、富集系数和转运系数均大于1.0,可用于植物修复污染土壤.     

三株甘蔗糖蜜酒精发酵高产酵母菌株的筛选

从甘蔗糖厂的废弃物中筛选到3株甘蔗糖蜜酒精发酵高产菌株MF1001、MF1002和MF1003,经rDNAITS序列同源比对鉴定为酿酒酵母(Saccharomyces cerevisiae)的不同菌株。3个菌株均可以完全利用葡萄糖和蔗糖,部分利用棉子糖和半乳糖,均不能利用乳糖和木糖。MF1001发酵甘蔗糖蜜的残糖含量明显低于目前使用的生产菌株,略低于标准测定菌株CICC31149和CICC31279,MF1002和MF1003的残糖含量略低于目前使用的生产菌株。与生产菌株相比,3个菌株在甘蔗糖蜜的生长速率略低,但是维持高菌数的时间较长。按目前甘蔗糖蜜酒精生产的发酵工艺,3个菌株30℃发酵72h的醪液酒精含量分别为14.26%(V/V)、14.48%(V/V)和13.50%(V/V),比目前生产使用的菌株高19.5%~28.6%;37℃发酵40h的醪液酒精含量分别为12.03%(V/V)、12.06%(V/V)和12.14%(V/V),比目前生产使用的菌株高10.0%~11.7%。这3个菌株具有提高甘蔗糖蜜发酵醪液酒精含量的潜在工业价值。     

一株产表面活性剂的菌株的筛选及现场试验研究

从胜利油田油水样中分离到一株高温条件下能够利用烃类产生表面活性剂的菌株A2,经鉴定属于Petrobacter.该菌株对两种原油的降解、降黏和降凝结果表明:对孤岛油的降解率较高,为39.81%,对甘17油的降解率达22.60%;对孤岛油的降黏率可达37.95%,对甘17油的降黏率为25.00%;A2可将孤岛油和甘17油的凝固点降低2.5℃.通过菌株作用岩石表面,表明该菌株对玻璃试片作用后其接触角降低最为明显,高达72%,接近于对照菌样的5倍;石英和灰岩的接触角也分别降低了68%和24%.表明A2具有一定的改变岩石表面性质的能力.增油效果评价实验结果表明:A2菌在中一驱Ng3油藏条件下,微生物驱提高采收率9.3%.现场实施后取得了降水增油效果,试验区含水下降2.1%,累积增油0.36×104t,提高采收率0.21%.     

Functional expression of mammalian opioid receptors in insect cells and high-throughput screening platforms for receptor ligand mimetics

Lepidopteran cell lines have been engineered to constitutively express high levels of mouse opioid receptors either alone or in combination with human G16 protein. Biochemical and pharmacological studies demonstrate that these lines contain all the mediator G proteins and downstream effectors required for opioid receptor function, including phospholipase C, and that expression of exogenous G16 does not contribute significantly to increased receptor responses upon activation. The activation of the phospholipase C pathway in the transformed cells upon stimulation with known receptor ligands results in easily and quantitatively measurable increases in free intracellular calcium, which can be monitored by automated fluorescent methods, while the addition of specific antagonists blocks the agonist-induced responses. Therefore, the transformed lepidopteran cell lines can be used as sensitive high-throughput screening platforms for fast detection of opioid receptor ligand mimetics (agonists and antagonists) in collections of natural products and synthetic compounds.Received 3 December 2004; received after revision 3 February 2005; accepted 10 February 2005L. Swevers and E. Morou contributed equally to this work.     

Physicochemical profiling in drug research: a brief survey of the state-of-the-art of experimental techniques

This review begins with a general presentation of the new paradigm of drug discovery, with its emphasis on the rapid identification and elimination of compounds with unsuitable physicochemical and pharmacokinetic properties. The focus of the paper is on the various experimental methods used to determine such key physicochemical properties as ionization, lipophilicity and distribution in isotropic and anisotropic systems, solubility, and permeability across artificial membranes. Both traditional and high-throughput methods are presented and their limits highlighted. The text concludes with the trade-off between quantity/speed in high-throughput screening techniques versus greater data quality in the more labor-intensive methods. Received 23 April 2002; received after revision 25 June 2002; accepted 11 July 2002 RID="*" ID="*"Corresponding author.     

Color image computer desktop printing system

Color image printing technique on ink jetting printer is introduced. Color shading method such as dithering, Amplitude Modulation (AM) screening and Frequency Modulation (FM) screening is presented. Some solutions to the problems with FM screening are given. An application program under Windows is developed. Supported by the International Cooperation Project (Japan Asahi High Tec Co. Ltd) Sun Xiaoan: born in July 1967, Ph. D